Background: Understanding nursing students' attitudes toward gender roles is vital for reducing gender-based discrimination and ensuring equitable and high-quality healthcare delivery. Purpose: This study aimed to assess nursing students' gender role attitudes of nursing students in the Southeastern Anatolia region of Turkey. Methods: This cross-sectional and descriptive study was conducted with 3244 nursing students using a convenience sampling method studying at universities in the southeastern region of Turkey. Data were collected using the Demographic Information Form and Gender Role Attitudes Scale. Results: It was observed that 61.0% of the nursing students obtained a score of < 95 points on the Gender Roles Attitude Scale. Nursing students' attitudes toward gender roles were traditional. A significant relationship was determined (R=0.456, R²=0.205) between students' socio-demographic variables and their attitudes toward gender roles (F=65.137, p<0.001). Gender role attitudes accounted for 20.5% of the variance in socio-demographic characteristics. Father's education level was the most significant predictor of influencing students' gender role attitudes (p<0.001). Conclusion: Nursing students' gender roles should be considered to prevent gender-based discrimination in healthcare services and to create a more equitable environment in the professional field. Implications for Nursing: Nursing education programs need to be revised to increase awareness of gender equality and roles. Implementing courses or training programs that focus on the impact of gender roles on healthcare can help students gain a better understanding of these roles.