Background: Nephrolithotomy is a major surgical procedure that often triggers significant anxiety in patients due to fear of pain, anesthesia, and surgical complications. Preoperative anxiety may adversely affect physiological stability and surgical outcomes. Recent technological advancements enable artificial intelligence (AI) tools, such as Meta AI on WhatsApp, to be utilized as interactive media for preoperative education. Objectives: This study sought to evaluate how effective preoperative education delivered through Meta AI was in reducing anxiety among patients scheduled for nephrolithotomy. Methods: A quasi-experimental, non-randomized pre-test and post-test control group design was employed. Seventy nephrolithotomy patients were purposively and non-randomly assigned to either an intervention group or a control group. The intervention group received preoperative education via Meta AI, whereas the control group received conventional verbal instruction. Anxiety levels were assessed using the Amsterdam Preoperative Anxiety and Information Scale (APAIS), and the data were analyzed with an independent samples t-test. Results: Both groups experienced a decline in anxiety after education; however, the intervention group showed a significantly greater reduction in anxiety scores (p = 0.001) compared to the control group. Conclusions: AI-based preoperative education through Meta AI effectively reduced preoperative anxiety among nephrolithotomy patients more than conventional verbal methods. Implications for Nursing: Nurses can integrate AI-based platforms into preoperative education to provide accessible, interactive, and patient-centered information that enhances psychological preparedness and reduces surgical anxiety.